Nano-object characterisation platform

The nano-object characterisation platform comprises two departments:

– microscopy and spectroscopy

– NTA, DLS and zetametry.

Microscopy and Spectroscopy Department

We have the following instruments at our disposal

– cryomicroscopy
– AFM
– X-RAY
– BET
– IR spectroscope
– UV spectroscope
– Fluorimeter

A nommer
A nommer

Nanometric measurements in solution

The equipment on this platform can be used to :

– characterise all types of nano-objects formulated within the research teams: size, distribution, zeta potential, etc.

– study the assembly mechanisms of these objects

– assess their stability in different environments

Operating procedures and training

The platform is located in room 2-845.

Training in the use of the equipment is compulsory for all new users.

Equipment

  • Zetasizer Nano ZS (Malvern Panalytical)

  • Zetasizer Ultra (Malvern Panalytical)

  • Nanosight LM10 (Malvern Panalytical)

The instruments in this platform enable the analysis of nano-objects using Dynamic Light Scattering (DLS) techniques.

The Zetasizer range allows determination of the size distribution of formulated nano-objects. The Zetasizer Ultra additionally performs measurements at multiple angles (MADLS), improving resolution between different populations potentially present within the same sample, and providing nanoparticle concentration for each population. Moreover, it is equipped with a fluorescence filter and both horizontal and vertical polarizers.

Furthermore, both Zetasizer instruments enable Electrophoretic Light Scattering (ELS), allowing measurement of electrophoretic mobility and zeta potential of nano-objects.

In contrast, the Nanosight LM10 uses Nanoparticle Tracking Analysis (NTA), a method that directly visualizes individual scattering nano-objects and provides their size distribution and concentration.

Julie MOUGIN
Julie MOUGINExperte en mesures nanoscopiques (IE)

Zetasizer Ultra (left) and Zetasizer Nano ZS (right)

Nanosight LM10